The dataset includes bat acoustic data recorded in sites administered by Parks Canada in the following regions of Quebec: Mauricie and Western Québec, Gaspésie, Québec, and Mingan. The protocols and methods used to collect and analyse the data may vary among sites and over time. The dataset includes all bat species recorded at each site between 2016 and 2022, and includes the following species : Eptesicus fuscus (EPFU), Myotis spp (MYSP), Myotis lucifugus (MYLU) , Eptesicus fuscus / Lasionycteris noctivagans (EPNO), Lasionycteris noctivagans (LANO), Lasiurus borealis (LABO), Lasiurus cinereus (LACI), Myotis septentrionalis (MYSE) as well as unidentified bats (SPP).