Permafrost is integral to the landscape of the Yukon, and influences hydrology and ecology, and impacts infrastructure. Accelerated permafrost thaw due to climate change poses significant challenges, particularly for the Alaska Highway, a vital transportation route. This study investigates how thawing permafrost may relate to ground instability, resulting in cracks and deformations along the highway. Thermal infrared imaging, and light detection and ranging (lidar) mounted on remotely piloted aircraft systems (RPAS), along with ground surveys, were completed at three localities along the Alaska Highway between the White River and the community of Beaver Creek, Yukon. Through these surveys, temperature variations and topographic changes were examined. At many locations, the damage is characterized by a 1 to 2 m-wide subsidence feature running longitudinally along the middle of the highway. Associated with these zones of subsidence are potholes, as well as longitudinal and transverse cracks. In places, the system of cracks extends to the edge and shoulder of the highway, suggesting that the cracks and deformation are currently active features.